Meconium
Bullet Blender® homogenization protocol

  • Extract molecules (DNA, RNA, protein, chemicals)
  • Wet final product
  • Sample sizes: 100 to 3500 mg.

Notes on the protocol: This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required

uL

One of these Bullet Blenders

  • Bullet Blender 50-DX (BB50-DX)
  • Bullet Blender 50 Gold (BB50-AU)

Reagents

Homogenization buffer
2 x volume of sample

Bead choices

  • 0.9 - 2.0 mm stainless steel blend (SSB14B) Use a volume of beads equivalent to 1 x the volume of the sample

Procedure

  1. Place the sample in the tube with the beads.
  2. Add a volume of buffer that is twice the volume of the sample. Sample volume may be approximated by sample weight. E.g., for a 100 mg. sample, add 0.2 ml. buffer.
  3. Close the tubes tightly and place them in the Bullet Blender.
  4. Set the controls for Speed 8 and Time 12. Press Start.
  5. After the run, remove the tubes from the instrument and visually inspect the samples. If homogenization is incomplete, repeat the homogenization step at a higher speed.
  6. Proceed with your downstream application.